wt sars cov 2 rbd  (Addgene inc)

Journal: Signal Transduction and Targeted Therapy

Article Title: Inducible CD147 up-regulation boosts extended SARS-CoV-2 infection triggering severe COVID-19 independent of ACE2

doi: 10.1038/s41392-025-02551-x

Figure Lengend Snippet: SARS-CoV-2-induced CD147 up-regulation causes extended virus infection and pathological injury. a A flow chart for establishing hCD147 mice model of COVID-19. b The virus loads in the lung tissues of hCD147 mice were detected by Taqman-based RT-PCR at 3 and 7 dpi, *** p < 0.001. c , d ,The expression of CD147 was evaluated by western blot in the lung tissues of virus-infected hCD147 mice at 3 and 7 dpi, and the lung tissues of healthy hCD147 mice were used as the control ( c ). The relative expression level of CD147 was analyzed by Image Lab software, *** p < 0.001 ( d ). e The scRNA-seq identified the expression of CD147 in virus-infected ( n = 4,203) and virus-uninfected ( n = 13,354) cells of lung tissues from hCD147 mice. Wilcoxon test, p < 0.0001. f The scRNA-seq identified the expression of CD147 in virus-infected and virus-uninfected AT2 cells of lung tissues from hCD147 mice. g –i Infected, border, and uninfected regions in the lung tissues of virus-infected hCD147 mice were defined using spatial transcriptomics ( g ). The total expression of CD147 was quantified in three defined regions ( h ), and the expression of CD147 in AT2, M2 macrophages, and fibroblasts was determined ( i ). The number of spots used for data analysis in uninfected, border, and infected group were 312, 3,554, and 1,833, respectively. Wilcoxon test, p < 0.05, p < 0.001. j The BEAS-2B cells with SARS-CoV-2 pseudovirus infection in the upper chamber were co-cultured with the BEAS-2B cells in the lower chamber for 48 h, and the expression of CD147 in cells collected from the lower chamber were detected by western blot. k , l The expression of CD147 was determined by western blot in BEAS-2B cells with SARS-CoV-2 pseudovirus infection ( k ) or RBD incubation ( l ). m The relative luciferase signals in pseudovirus-infected BEAS-2B cells were determined by dual-luciferase reporter assays with RBD incubation, *** p < 0.001. n The relative luciferase signals in pseudovirus-infected BEAS-2B cells were determined by dual-luciferase reporter assays, ns, not significant, *** p < 0.001. o The virus loads in authentic virus-infected BEAS-2B cells were determined by Taqman-based RT-PCR, ns, not significant, ** p < 0.01, *** p < 0.001. p A flow chart for establishing a virus-infected hCD147 mouse model by primarily inoculating with RBD protein followed by authentic virus infection. q The expression of CD147 was detected in the lung tissues of hCD147 mice with His-RBD ( n = 4) or His-tag ( n = 4) inoculation and hCD147 mice with His-RBD ( n = 4) or His-tag ( n = 4) inoculation followed by authentic virus infection. r The virus loads in the lung tissues of virus-infected hCD147 mice were determined, * p < 0.05. s , t The infiltration of F4/80+iNOS+ M1 macrophages and Ly6G+ neutrophils in lung tissues of virus-infected hCD147 mice was evaluated by multicolor immunofluorescence staining, Ly6G, yellow; F4/80, green; iNOS, red, scale bar, 100 μm ( s ), and their infiltrated percentages were quantified, ** p < 0.01, *** p < 0.001 ( t ). u The level of cytokines and chemokines in lung tissues of virus-infected hCD147 mice was performed by RT-PCR, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The mobile phases contained the mutants of CD147 (His-CD147-R54A, His-CD147-E84A, His-CD147-E92A, His-CD147-Q100A, His-CD147-S112A, our laboratory), the mutants of RBD (His-RBD-G413A, His-RBD-K417A, His-RBD-K424A, His-RBD-G447A, His-RBD-Y489A, Sino Biological), His-RBD (WT) (40592-V08H, Sino Biological), His-RBD (Beta) (40592-V08H85, Sino Biological), His-RBD (Gamma) (40592-V08H86, Sino Biological), His-RBD (JN.1) (40592-V08H155, Sino Biological), rhesus macaque CD147 (Sino Biological), human ACE2 (Sino Biological), and rhesus macaque ACE2 (Sino Biological).

Techniques: Virus, Infection, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Control, Software, Cell Culture, Incubation, Luciferase, Multicolor Immunofluorescence Staining

Journal: Emerging Microbes & Infections

Article Title: Prolonged Omicron-specific B cell maturation alleviates immune imprinting induced by SARS-CoV-2 inactivated vaccine

doi: 10.1080/22221751.2024.2412623

Figure Lengend Snippet: Memory B cell responses after an extended period post-BA.5 infection. (A) Timeline of vaccination, infection, and blood draws for 3 human cohorts, including short-term post-BA.5/BF.7 BTI sampling at 1 month (BA.5/BF.7 BTI (1 m)), prolonged post-BA.5/BF.7 BTI sampling at 7 months (BA.5/BF.7 BTI (7 m)), and prolonged post-BA.5/BF.7 infection sampling at 8 months (BA.5/BF.7 inf. (8 m)). (B) Flow cytometry analysis of pooled memory B cells from three cohorts including BA.5 (1 m), BA.5/BF.7 BTI (7 m), and BA.5/BF.7 inf. (8 m). BA.5 RBD APC and PE double-positive memory B cells (left panel) were analyzed for cross-reactivity with the wide-type (WT) RBD (right panel).

Article Snippet: Additionally, 0.013 μg of BA.5 RBD (Sino Biological, 40592-V49H9-B) tagged with PE-streptavidin (Biolegend, 405204) and APC-streptavidin (Biolegend, 405207), and 0.013 μg of WT RBD (Sino Biological, 40592-V27H-B) tagged with BV605 streptavidin (Biolegend, 405229) was added.

Techniques: Infection, Sampling, Flow Cytometry

Journal: Cell reports

Article Title: Beta-spike-containing boosters induce robust and functional antibody responses to SARS-CoV-2 in macaques primed with distinct vaccines

doi: 10.1016/j.celrep.2023.113292

Figure Lengend Snippet: A subunit booster improves antibody profiles in both macaques primed with two-dose mRNA vaccines and macaques primed with two-dose subunit vaccines (A) Schematic representation of the cohorts: in the mRNA-primed cohort, 16 macaques were evenly split into four groups, each group was primed with mRNA vaccines in different modalities on days 0 and 21 and boosted with a subunit booster on day 210, and blood samples were collected 2 weeks after the second primary series on day 35 and on days 205 and 224. In the protein-primed cohort, 24 macaques were split into five groups, each group was primed with subunit vaccines in different modalities on days 0 and 21 and boosted with a subunit booster on day 202, and blood samples were collected 2 weeks after the second primary series vaccination on day 35 and on days 196 and 216. (B) The dot plots show the SARS-CoV-2 ancestral spike-specific IgG1 titer and the ability of the spike-specific antibodies to bind to the low-affinity Fcγ receptors (FcγRIIA and FcγRIIIA) across the macaques primed with mRNA vaccines and boosted with a subunit booster in mRNA prime (top) at different time points: peak (day 35), pre-boost (day 205), and post-boost (day 224), and the macaques primed with subunit vaccines and boosted with a subunit booster in protein prime (bottom) at different time points: peak (day 35), pre-boost (day 205), and post-boost (day 224). Each dot represents a different macaque. Each bar represents the median of each group. (C) The dot plots show antibody-dependent cellular phagocytosis (ADCP), antibody-dependent neutrophil phagocytosis (ADNP), antibody-dependent complement deposition (ADCD), and antibody-dependent natural killer (NK) cell activity (ADNK), as measured by CD107a degranulation, against SARS-CoV-2 ancestral spike in macaques primed with mRNA vaccines and boosted with a subunit booster in mRNA prime (top) at different time points: peak (day 35), pre-boost (day 205), and post-boost (day 224), and the macaques primed with subunit vaccines and boosted with a subunit booster in protein prime (bottom) at different time points: peak (day 35), pre-boost (day 196), and post-boost (day 216). *p < 0.05 against the no-AS03 post-boost group, ns, not significant.

Article Snippet: SARS-CoV-2 WT RBD , Sino Biological , 40592-V08H.

Techniques: Vaccines, Activity Assay

Journal: Cell reports

Article Title: Beta-spike-containing boosters induce robust and functional antibody responses to SARS-CoV-2 in macaques primed with distinct vaccines

doi: 10.1016/j.celrep.2023.113292

Figure Lengend Snippet: IgA toward SARS-CoV-2 spikes is expanded by Beta-containing component boosters in protein-primed NHPs (A) Mean IgA binding to indicated SARS-CoV-2 VOCs in mRNA-primed NHPs. Shown are the values after the primary series, pre-boost, and post-boost against the indicated VOC spike or tetanus as a negative control. Shown on the y axis is the IgA mean fluorescent intensity (MFI) of binding to the antigen, and on the right is the color scheme. (B) Same as (A) but for NHPs who received a protein primary vaccines series. Statistical significance (Mann-Whitney U test) of peak responses after primary series and post-boost was determined to show expansions of IgA binding breadth. *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: SARS-CoV-2 WT RBD , Sino Biological , 40592-V08H.

Techniques: Binding Assay, Negative Control, Vaccines, MANN-WHITNEY

Journal: Cell reports

Article Title: Beta-spike-containing boosters induce robust and functional antibody responses to SARS-CoV-2 in macaques primed with distinct vaccines

doi: 10.1016/j.celrep.2023.113292

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SARS-CoV-2 WT RBD , Sino Biological , 40592-V08H.

Techniques: Recombinant, Virus, Software, Labeling, Luminex

Journal: iScience

Article Title: Placental transfer dynamics and durability of maternal COVID-19 vaccine-induced antibodies in infants

doi: 10.1016/j.isci.2024.109273

Figure Lengend Snippet: Maternal vaccine-induced SARS-CoV-2 antibodies persist in 2-month and 6-month infants Dot plots showing (A) Spike-specific WT (top) and RBD-specific WT antibody levels and (B) FcR profiles of maternal (M), cord (C), 2-month-old infants (2 m), and 6-month-old infants (6 m). Significance was determined by a Kruskal-Wallis test followed by Benjamini-Hochberg procedure for multiple hypotheses correction. If statistically significant, a two-sided Mann-Whitney U test was performed. (∗p < 0.05, ∗∗p < 0.01,∗∗∗p < 0.001,∗∗∗∗p < 0.0001). The dashed line represents the level of detection for each feature, defined as one standard deviation above the mean value for assay negative biological controls (SARS-CoV-2 negative, unvaccinated samples).

Article Snippet: SARS-CoV-2 RBD WT , Sino Biological , CAT # 40592-V08H.

Techniques: MANN-WHITNEY, Standard Deviation

Journal: iScience

Article Title: Placental transfer dynamics and durability of maternal COVID-19 vaccine-induced antibodies in infants

doi: 10.1016/j.isci.2024.109273

Figure Lengend Snippet: Maternal vaccine timing drives IgG levels and Fc-receptor binding profiles in 2-month infants Infants whose mothers were vaccinated in the late second and early third trimester (received at least one dose of mRNA vaccine after 28 weeks) had the highest levels of antibodies at 2 months. (A) Diagram showing the grouping strategy for 2-month-old infants based on the dose 1 (D1) and dose 2 (D2) COVID-19 vaccination. Group 1: N = 12 , Group 2: N = 11, Group 3: N = 10, Group 4: N = 6, Group 5: N = 8. (B) Univariate plots showing Spike-specific WT antibody and Fc-receptor (FcR) levels of each 2-month-old infant group (as shown in in A) Significance was determined by a Kruskal-Wallis test followed by Benjamini-Hochberg procedure for multiple hypotheses correction. If statistically significant, a two-sided Mann-Whitney U test was performed. (∗p < 0.05, ∗∗∗∗p < 0.0001). The middle line represents the median, whereas the other lines represent the first and third quartiles. The dashed line represents the level of detection for each feature, defined as one standard deviation above the mean value for assay negative biological controls (SARS-CoV-2 negative, unvaccinated samples). (C) (Top) A partial-least squares discriminant model (PLSDA) was built using LASSO feature regularization and variable selection of the infant cohort group, resulting a reduced set of features that were consistently in at least 80 of the 100 LASSO models. (Bottom) Variable importance in projection (VIP) score of the selected features. The magnitude indicates the importance of the features in driving the separation in the model. The color of the feature corresponds to the group to which the feature was enriched.

Article Snippet: SARS-CoV-2 RBD WT , Sino Biological , CAT # 40592-V08H.

Techniques: Binding Assay, MANN-WHITNEY, Standard Deviation, Selection

Journal: iScience

Article Title: Placental transfer dynamics and durability of maternal COVID-19 vaccine-induced antibodies in infants

doi: 10.1016/j.isci.2024.109273

Figure Lengend Snippet: Maternal vaccine administration at >32 weeks is associated with reduced antibody persistence in 6-month infants (A) The polar plots depict the mean percentile ranks of the antibody features within 6 months infants whose mothers were vaccinated with the first dose of the mRNA COVID-19 vaccine <20 weeks ( N = 20), 20–28 weeks ( N = 26), and >28 weeks ( N = 30). Each wedge represents an antibody feature, and the size of the wedge depicts the mean percentile ranging from 0 to 1. (B) Linear regression models fit the relationship between gestation age of dose 1 (days) (those vaccinated between 28 and 32 weeks) and anti-RBD WT IgG1, anti-S1 FcγR2AR, and anti-RBD Gamma FcγR3B. The rho and p values are reported in the plot. The dashed line represents the level of detection for each feature, defined as one standard deviation above the mean value for assay negative biological controls (SARS-CoV-2 negative, unvaccinated samples). (C) Linear regression models fit the relationship between gestation age of dose 1 (days) (those vaccinated between 32 and 36 weeks) and anti-RBD WT IgG1, anti-S1 FcγR2AR, and anti-RBD Gamma FcγR3B. The rho and p values are reported in the plot. The dashed line represents the level of detection for each feature, defined as one standard deviation above the mean value for assay negative biological controls (SARS-CoV-2 negative, unvaccinated samples). (D) The heatmap shows the Spearman correlation score for each feature against gestational age (days) within infant samples whose mothers received the first dose of the mRNA COVID-19 vaccine between 28 and 32 weeks or 32–36 weeks. The red boxes indicate samples with a positive Spearman correlation and blue boxes indicate samples with a negative Spearman correlation. Within each group, gray boxes are features which were excluded from the analysis (if >70% of values fell below the threshold of detection, defined as less than one standard deviation above the mean of SARS-CoV-2 negative, unvaccinated control samples).

Article Snippet: SARS-CoV-2 RBD WT , Sino Biological , CAT # 40592-V08H.

Techniques: Standard Deviation

Journal: iScience

Article Title: Placental transfer dynamics and durability of maternal COVID-19 vaccine-induced antibodies in infants

doi: 10.1016/j.isci.2024.109273

Figure Lengend Snippet: IgG subclasses and Fc-receptor binding capacity of antibodies against SARS-CoV-2 variants of concern in umbilical cord blood, 2-month, and 6-month infants (A) The heatmap shows the ratio of infants with detectable antibody titer or FcR binding levels N = 76 for cord (C), N = 55 for 2-month-old infants (2 M), and N = 80 for 6-month-old infants (6 M) against the Spike or RBD region of wild-type (WT) strain and variants of concern (VOCs) including Alpha, Beta, Delta, Gamma, and Omicron. (B) Univariate plots showing RBD-specific Omicron antibody IgG levels (top) and FcR binding profiles in cord (C), 2-month-old infants (2 m), and 6-month-old infants (6 m). Significance was determined by a Kruskal-Wallis test followed by Benjamini-Hochberg procedure for multiple hypotheses correction. If statistically significant, a two-sided Mann-Whitney U test was performed. (∗p < 0.05, ∗∗p < 0.01,∗∗∗p < 0.001,∗∗∗∗p < 0.0001). The dashed line represents the level of detection for each feature, defined as one standard deviation above the mean value for assay negative biological controls (SARS-CoV-2 negative, unvaccinated samples).

Article Snippet: SARS-CoV-2 RBD WT , Sino Biological , CAT # 40592-V08H.

Techniques: Binding Assay, MANN-WHITNEY, Standard Deviation

Journal: iScience

Article Title: Placental transfer dynamics and durability of maternal COVID-19 vaccine-induced antibodies in infants

doi: 10.1016/j.isci.2024.109273

Figure Lengend Snippet:

Article Snippet: SARS-CoV-2 RBD WT , Sino Biological , CAT # 40592-V08H.

Techniques: Recombinant, Produced, Generated, Software, Luminex